tirf 3 microscope Search Results


laser tirf 3  (Cleaver Scientific)
90
Cleaver Scientific laser tirf 3
Laser Tirf 3, supplied by Cleaver Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tirf+3+microscope/the+laser+tirf+3+is+a+total+internal+reflection+fluorescence++tirf++microscopy+system/10__1126_slash_science__324_1735a-27-9-5
Average 90 stars, based on 1 article reviews
laser tirf 3 - by Bioz Stars, 2026-10
90/100 stars
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tirf 3 axio observer setup  (Carl Zeiss)
99
Carl Zeiss tirf 3 axio observer setup
Tirf 3 Axio Observer Setup, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tirf+3+microscope/Microscope+stand+Axio+Observer+3/pmc08563363-787-12-11
Average 99 stars, based on 1 article reviews
tirf 3 axio observer setup - by Bioz Stars, 2026-10
99/100 stars
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tirf microscope  (Carl Zeiss)
96
Carl Zeiss tirf microscope
a,b , Representative PLA signal (red dots) (a) and quantification of the distribution of the PLA signal relative to the total cell area (F-actin) (b) revealing more co-localization between talin and α5-integrin following SHANK3 silencing in HEK293 cells. Data are displayed as Tukey box plots and expressed relative to control-silenced cells (n = 72 cells from three independent experiments). c , β1-integrin (P5D2) immunoprecipitation showing enhanced interaction between β1-integrin and talin following SHANK3 silencing in HEK293 cells (representative blot from four independent experiments is shown). d,e , MDA-MB-231 cells transiently expressing GFP-Talin1 were plated on fibronectin-collagen and imaged live using a <t>TIRF</t> <t>microscope</t> (1 picture every 1 min for more than 3 h; scale bar = 20 μm (original image) and 10 µm (ROI)) (d). The percentage of cells displaying a “talin wave” was then counted (three biological repeats; 84 siCTRL and 86 siSHANK1 movies analysed) (e). f,g , MDA-MB-231 cells transiently expressing mEmerald-Paxillin were plated on fibronectin-collagen and imaged live using a TIRF microscope (1 picture every 1 min for more than 3 h; scale bar = 20 μm) (f). Focal adhesion lifetime was analysed using the focal adhesion analysis server (see ) (two biological repeats; over 33 movies per condition analysed; n = 97817 siCTRL and 164092 siSHANK1 adhesions analysed) (g). Tukey box plots represent median and 25 th and 75 th percentiles (interquartile range); points displayed as outliers if 1.5 times above or below the interquartile range; outliers are represented by dots. Statistical analysis: Student’s t-test. Unprocessed original scans of blots are shown in .
Tirf Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tirf+3+microscope/Optovar+1%2E6x/pmc05386136-300-3-5
Average 96 stars, based on 1 article reviews
tirf microscope - by Bioz Stars, 2026-10
96/100 stars
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Image Search Results


a,b , Representative PLA signal (red dots) (a) and quantification of the distribution of the PLA signal relative to the total cell area (F-actin) (b) revealing more co-localization between talin and α5-integrin following SHANK3 silencing in HEK293 cells. Data are displayed as Tukey box plots and expressed relative to control-silenced cells (n = 72 cells from three independent experiments). c , β1-integrin (P5D2) immunoprecipitation showing enhanced interaction between β1-integrin and talin following SHANK3 silencing in HEK293 cells (representative blot from four independent experiments is shown). d,e , MDA-MB-231 cells transiently expressing GFP-Talin1 were plated on fibronectin-collagen and imaged live using a TIRF microscope (1 picture every 1 min for more than 3 h; scale bar = 20 μm (original image) and 10 µm (ROI)) (d). The percentage of cells displaying a “talin wave” was then counted (three biological repeats; 84 siCTRL and 86 siSHANK1 movies analysed) (e). f,g , MDA-MB-231 cells transiently expressing mEmerald-Paxillin were plated on fibronectin-collagen and imaged live using a TIRF microscope (1 picture every 1 min for more than 3 h; scale bar = 20 μm) (f). Focal adhesion lifetime was analysed using the focal adhesion analysis server (see ) (two biological repeats; over 33 movies per condition analysed; n = 97817 siCTRL and 164092 siSHANK1 adhesions analysed) (g). Tukey box plots represent median and 25 th and 75 th percentiles (interquartile range); points displayed as outliers if 1.5 times above or below the interquartile range; outliers are represented by dots. Statistical analysis: Student’s t-test. Unprocessed original scans of blots are shown in .

Journal: Nature cell biology

Article Title: SHANK proteins limit integrin activation by directly interacting with Rap1 and R-Ras

doi: 10.1038/ncb3487

Figure Lengend Snippet: a,b , Representative PLA signal (red dots) (a) and quantification of the distribution of the PLA signal relative to the total cell area (F-actin) (b) revealing more co-localization between talin and α5-integrin following SHANK3 silencing in HEK293 cells. Data are displayed as Tukey box plots and expressed relative to control-silenced cells (n = 72 cells from three independent experiments). c , β1-integrin (P5D2) immunoprecipitation showing enhanced interaction between β1-integrin and talin following SHANK3 silencing in HEK293 cells (representative blot from four independent experiments is shown). d,e , MDA-MB-231 cells transiently expressing GFP-Talin1 were plated on fibronectin-collagen and imaged live using a TIRF microscope (1 picture every 1 min for more than 3 h; scale bar = 20 μm (original image) and 10 µm (ROI)) (d). The percentage of cells displaying a “talin wave” was then counted (three biological repeats; 84 siCTRL and 86 siSHANK1 movies analysed) (e). f,g , MDA-MB-231 cells transiently expressing mEmerald-Paxillin were plated on fibronectin-collagen and imaged live using a TIRF microscope (1 picture every 1 min for more than 3 h; scale bar = 20 μm) (f). Focal adhesion lifetime was analysed using the focal adhesion analysis server (see ) (two biological repeats; over 33 movies per condition analysed; n = 97817 siCTRL and 164092 siSHANK1 adhesions analysed) (g). Tukey box plots represent median and 25 th and 75 th percentiles (interquartile range); points displayed as outliers if 1.5 times above or below the interquartile range; outliers are represented by dots. Statistical analysis: Student’s t-test. Unprocessed original scans of blots are shown in .

Article Snippet: Images with a TIRF microscope (Zeiss Laser-TIRF 3 Imaging System, Carl Zeiss) were acquired using a 10x objective (NA 0.25 air) or a 63x (NA 1.46 Oil, alpha Plan-Apochromat, DIC) objective with an internal Optovar (1.6x magnification) and an EMCCD camera (Hamamatsu ImageEM C9100-13; Chip size 512x512; Hamamatsu Photonics K.K., Hamamatsu City, Japan).

Techniques: Control, Immunoprecipitation, Expressing, Microscopy